| Main scientific achievements: | - A number of bioinformatic analysis tools distinguished by their high accuracy (~ 86-95%) for searching for gene boundaries (transcription start sites and primary mRNA polyadenylation sites) in human, animal, plant and bacterial genomes - PromH, PromHP, TSSP-TCM, POLYAR, Pparf, bTSSfinder, and TSShm programs, computer search programs for transcription regulatory elements-nsite, nsiteM and nsiteH, and PlantProm database on RNA polymerase II promoters of plants were created.
- In the nuclear genomes of a number of higher plants (Arabidopsis, rice, wine grape, etc.), the spectrum and extent of distribution of DNA sequences of plastid and mitochondrial origin was clarified, facts indicating the expression of intact nuclear copies of some plastid and mitochondrial genes were detected.
- The possibility of generating potential chimeric RNAs and proteins induced by transcription in humans, some mammals, and higher plants was explored. It was found that each of these organisms has numerous pairs of close (several hundred nucleotide pairs) neighboring protein gene pairs located "Head-Tail" in the nuclear genome. They have the potential to generate chimeric mRNAs by co-transcription and alternative splicing, as well as chimeric polypeptides if translation occurs.
- Characteristics of the relative location of protein and long non-coding RNA (ukRNA) genes on the chromosome were investigated in humans, rice, maize, Arabidopsis, soybean, alfalfa, black poplar, cultivated tomatoes, potatoes and wine grapes. The presence of ukRNA genes located in the close neighborhood of many protein genes, on the same or opposite strands of DNA, or intersecting with them was discovered in each of those organisms.
- Searching for potential novel open reading frames in known mRNA sequences of human, rhesus monkey, chimpanzee, house mouse, Norway rat, rice, maize, soybean, alfalfa, black poplar, cultivated tomato, and wine grape revealed that each of these species has at least some mRNA sequences having the potential to encode additional proteins. These results suggest that the proteome of these organisms is much larger than is currently known.
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