Nurmammad Shamil Mustafayev 
| Place of birth | Azerbaijan Republic, Sheki city |
| Date of birth | 10.10.1965 |
| Education | Azerbaijan State University |
| Scientific degree | PhD in Biology |
| Title | Associate Professor |
| Specialty code and title for PhD thesis | 2409.01 Genetics “Some regulatory elements of structural-functional organization of eukaryotic genomes” |
| Total number of printed scientific publications | 41 |
| Number of scientific publications printed abroad | 17 |
| Number of papers published in journals indexed and abstracted in international databases | 10 |
| Number of patents and certificates of authorship | none |
| Staff training: - number of PhDs |
Supervision of 3 PhD students and 4 masters (2 of them already defended) |
| Basic scientific achievements | 5'-promoter regions of all the protein and rRNT coding genes of plastid (chloroplast) genomes of three higher plants (rice - Oryza sativa, corn - Zea mays and tobacco - Nicotiana tabacum) for the first time have been analyzed by computer programs, it has been found that these regions are rich with the different types and numerous statistically nonrandom NEP (nuclear encoded promoter) promoter elements and motifs of different regulation elements. At present the studies with aim to obtain promoter (TATA and non-TATA) maps of the nucleus and chloroplast genomes of higher plants (currently 7 higher plants), full genomes of which were sequenced completely are still underway. A comparative analysis of the transcription factors bounding DNA domains that regulate the expression of chloroplast related nuclear gene groups in several higher plants (Arabidopsis, rice, corn, black pine etc.) having full-genomic sequence, experimentally testing of functionality of the some found potential transcription regulating elements (TE) and studies of possible molecular mechanisms of coordinated and cooperative expression of nuclear-plastid gene regulation at the transcriptional level are also being carried out. By using analogous computer methods some minisatellites of human and Arabidopsis have been studied, and richness of these sequences by numerous potential transcription and replication regulatory elements has been demonstrated. One of the key performers in creating of PlantProm database (http://www.rhul.ac.uk/; http://www.softberry.com; http://cub.comsats.edu.pk) of the transcribed by RNA polymerase II plant genes promoters the transcription start site (TSS) of which were experimentally found.
In addition, for the first time Azerbaijan population were studied by using of markers consistinf of short tandem repeats (STRs) located on the autosomal and sex chromosomes and the following results were obtained:
Study of our population with STR markers located on autosomal chromosomes., DNA samples isolated from 765 unrelated Azerbaijanis covering all regions and living in Azerbaijan for several generations were studied with STR markers (human identification kit, AmpFlSTR®Identifiler®Plus PCR Amplification Kit) located on autosomal chromosomes. As a result of the study, population-genetic and forensic-medical parameters were determined for each STR locus, P values of accuracy of the tests (PHWE) were calculated on the basis of Hardy-Weinberg equation, and combined parameters (CPE, CPD, CTPI and PP) were calculated based on the STR loci set. It has been shown that this set of markers is suitable for use in solving identification problems in our population. In other words, these fundamental results can be applied in the practice of forensic medicine (identification, disputed paternity/mothernity, etc.). In addition, multiplet differentiation tests were performed between the Azerbaijani population and 17 world populations, and the P-values of the Fisher genetic distance (FST) indices were compared. Genetic testing revealed no statistically significant differences in all STR loci studied between the populations of Iraq, Iran, and Turkey. Statistically significant differences were found in distant and concerning to other races populations (China, South Africa, Bolivia, etc.). Principle component (PCA) and principal coordinate (PcoA) analyzes based on FST values showed that the Azerbaijan population was grouped with different populations in different quarters of the coordinate system, with a negative and zero correlation, respectively. A comparison of phylogenetic trees constructed by two mathematical methods (NJ and UPGMA) based on Nei’s genetic distances shows that a tree constructed with the UPGMA approach is more realistic. The results of the study were published in 2020 in the Gene journal (IF=2,984).
Study of our population with STR markers located on sex chromosomes. Within the framework of the grant project entitled “THE STUDY OF GENE FUND OF AZERBAIJAN POPULATION BASED ON STR-MARKERS OF Y-CHROMOSOME” won in the international grant competition announced by the Ukrainian Science and Technology Center (STCU) for 225 Azerbaijani citizens living in Azerbaijan Y-chromosome haplotypes using AmpFlSTR®Yfiler®PCR Amplification Kit consisting of 16 markers were obtained, haplogroups of these individuals were identified through two different haplogroup predictors, and a distribution map of the haplogroups was compiled. It was found that the in the formation of the Azerbaijani population gene pool main role play Middle Eastern, Caucasian and European origin E, G, J, R and T haplogroups and their subclades (~85%). In addition, for the revealed haplotypes a number of population-genetic and forensic parameters (allele frequencies, haplotype diversity, matching probability, discriminatory power, polymorphic information content, typical paternity index, etc.) were determined, and these parameters shown to be valid of these markers for use in solving of identification problems. In addition, RST genetic distance indices and their P-values were calculated with the help of YHRD-based programs, and on the basis of these values, our population was comparatively analyzed with related, geographically close and distant populations. It was found that our population is closer to the populations of Turkey, Iraq and Iran in terms of Y-DNA structure. These results once again confirm the results obtained with autosomal markers. The results for haplotype diversity are placed in a local (http://imbb.az/uploads/AZE_Y_STR_Profiles _for_YHRD.pdf) and international (https://yhrd.org/YA00469) databases with accession number YA00469. Before us, there were only 47 Y-DNA profiles in the international database, which were obtained mainly by foreign experts in Moscow and Germany. In this sense, the 225 profiles posted on the database without the participation of external experts can be considered a great success for Azerbaijan. It should be noted that research in this direction will be continued using a network with a wider Y-STR marker.
One of the important directions of population research is the study of hereditary and non-inherited diseases at the population level, which are widespread in the population. The sudden outbreak of the COVID-19 pandemic also proved how important this is. In view of the above, a preliminary study of lung and cardiovascular pathologies, neurodegenerative disorders and diabetes depend on genetic factors has been started in this direction, and the following results have been obtained during the initial research in this direction: Study of hereditary and non-hereditary diseases at the population level. The study of identification of different types of mutations (insertiion/deletion, SNP, etc.) with molecular markers, which are the main cause of hereditary and non-hereditary diseases have great importance. For this purpoze, to determine the relationships of detected mutant allelic forms to diseases, the insertion/deletion (I/D) polymorphism was studied in 346 non-relative persons of different ages and working in different occupations. Note that the receptor for the ACE gene has a special role in the spread of the coronavirus, as the virus enters the cell through the receptors of this enzyme and begins to multiply. Six female persons with II genotype were found in the group of conditionally healthy individuals, and three women and six men were found among mental patients with different diagnoses. Although one female with genotype II was found among patients with type 1 diabetes, no genotype II was found within the group, which consisted entirely of male athletes. The study revealed that the D allele predominated among the detected alleles, and the ID genotype predominated among the genotypes, confirming the literature data on the direct involvement of the D allele as a genetic factor in the development of pathologies within the studied groups (odds ratio for the D allele OR=1.6513; 95% CI=1.1509¸2.3694; Z-statistic = 2.7230; P=0.0065). Diabetes. We have launched a molecular-genetic study of hereditary predisposition to various forms of diabetes mellitus (DM) in Azerbaijan. At the initial stage, the association of T-cell transcription factor gene (TCF7L2) SNP type C/T rs7903146 mutations with insulin-dependent (28 patients) and non-insulin dependent (72 patients) DM was studied. Examination of dominant (D) and recessive (R) models with C and T alleles, both in individual groups and in the population, did not reveal significant differences in their ratios. These ratios are for the population: CD:CR=11.22; TD:TR=11.33; CD:TD=1.53; CR:TR=1.54. However, in the statistical evaluation of the relationship of the T allele to type II DM in the 95% confidence interval (CI) is 95%CI=0.8772¸5.8179; odds ratio OR=2.2604, Z-statistic=1.6910 and significance level P=0.0909. This consistent with existing ideas in literature on the role of the T allele as a genetic factor in the formation of type II DM. Analysis of the experimental results shows that although the frequency of the T allele and the number of TT homozygotes are higher in group II DM than in the control group, the statistically significant correlation between TCF7L2 gene rs7903146 C/T polymorphism and type II DM as a risk factor is lower than expected (p<0.1). This seems to be primarily due to the limited size of the sample involved in the study. |
| Names of scientific works | 1. Mammadov E.R., Mustafayev N.Sh., Mammadov A.Ch., Hasanov A.B., Huseynova I.M. Population-genetic and comparative interpopulation studies of Azerbaijan population based on the 15 autosomal STR markers // Gene, 2020, v. 753, Article ID 144804. https://doi.org/10.1016/j.gene.2020.144804; IF=2,984 2. Mustafayev N.Sh., Mammadov E.R., Mammadov A.Ch., Gasimova F.I., Rustamova Z.R., Hasanov A.B., Karimov Z.M., Farajova E.A. Y-DNA Polymorphism of Azerbaijan population: Preliminary studies // Proc. of Int. Conf. “Human genetics and Genetic Diseases: Problems and development prospects”, 2020, p. 50. 3. Rustamova Z.R., Akhundova L.A., Alibayova G.R., Mustafayev N.Sh., Huseynova I.M. Association of rs7903146 C/T polymorphism of TCF7L2 gene with type 2 diabetes mellitus in Azerbaijan population: Preliminary studies // Proc. of Int. Conf. “Human genetics and Genetic Diseases: Problems and development prospects”, 2020, p. 71. 4. Mammadov E.R., Mustafayev N.Sh., Mammadov A.Ch., Hasanov A.B., Huseynova İ.M. Mutation cases in the paternity tests identified using 15 autosomal STR markers // Proc. of Int. Conf. “Human genetics and Genetic Diseases: Problems and development prospects”, 2020, p. 51. 5. Mammadov E.R., Mustafayev N.Sh. Study of polymorphism in Azerbaijan population based on short tandem repeats (STR) // Proceedings of 2nd International Conference on "One Health: Problems & Solutions, 2020. 6. Алибейли Г.Р., Ахундова Л.А., Мустафаев Н.Ш., Гусейнова И.М. Исследование инсерционно-делеционного (I/D) полиморфизма гена ангиотензин-превращающего фермента (АПФ) среди населения Азербайджана / Материалы международной научно-практической конференции «Молекулярная диагностика и биобезопасность – 2020». Россия: Москва, 2020, 1 с. 7. Akhundova L.A., Rustamova Z.R. Some polymorphic genetic markers associated with type 1 and type 2 diabetes. Proc. of the 1st International Scientific Conferences of Students and Young Researchers dedicated to the 97th birthday anniversary of National Leader Heydar Aliyev, Baku, 2020, p. 77-79. 8. Mustafayev N.Ş. COVID-19: Xroniki və autoimmun xəstələr üçün təhlükə mənbəyidir / “Koronavirus pandemiyasi: Elmi tədqiqatlardan sağlam gələcəyin təminatına doğru” Beynəlxalq onlayn konfransın materialları, Bakı, 2020, s. 64-65. 9. Əlibəyova G.R., Rüstəmova S.M., Mustafayev N.Ş., Hüseynova İ.M. SARS-CoV-2 və renin-angiotenzin-aldosteron sistemi arasında qarşılıqlı əlaqə / “Koronavirus pandemiyasi: Elmi tədqiqatlardan sağlam gələcəyin təminatına doğru” Beynəlxalq onlayn konfransın materialları, Bakı, 2020, s. 21-22. 10. Mustafayev N.Ş., Məmmədov E.R., Məmmədov Ə.Ç., Nəsibov E., Qasımova F.I., Rüstəmova Z.R., Həsənov Ə.B., Kərimov Z.M., Fərəcova E.A. Azərbaycan əhalisində Y-xromosom DNT-nin polimorfizmi / “Koronavirus pandemiyasi: Elmi tədqiqatlardan sağlam gələcəyin təminatına doğru” Beynəlxalq onlayn konfransın materialları, Bakı, 2020, s. 122-123. 11. Mustafayev N.Sh., Mammadov E.R., Mammadov A.Ch., Hasanov A.B., Huseynova I.M. Mutation cases in the paternity tests using 15 autosomal STR markers // Journal of Life Sciences & Biomedicine, 2019, vol. 1(74), No 1, p. 5-17 12. Mustafayev N.Sh., Mammadov E.R., Huseynova F.R., Mammadov A.Ch., Hasanov A.B., Huseynova I.M. Population-genetic data For 15 Autosomal STR markers and comparative interpopulation studies of Azerbaijan population based on these data / 2nd International TURAZ ACADEMY Forensic Sciences, Forensic Medicine and Pathology Congress (ITAC, Science and Medicine). Turkey: Istanbul, 2018, SS-112, p. 201-202. 13. Huseynova I.M., Mirzayeva S.M., Sultanova N.F., Aliyeva D.R., Mustafayev N.Sh., Aliyev J.A. Virus-induced changes in photosynthetic parameters and peroxidase isoenzyme contents in tomato (Solanum lycopersicum L.) plants // Photosynthetica, 2018, v. 56, p. 841-850. 14. Mustafayev N.Sh., Mammadov E.R., Huseynova F.R., Mammadov A.Ch., Balakishiyeva G., Hasanov A.B., Huseynova I.M. Population-genetic parameters of the 15 autosomal STR markers for Azerbaijan population / The abstracts of the 23rd Annual WAML World Congress. Baku: 2017 // Medicine and Law, v. 36, No 2, p. 286. 15. Mustafayev N.Ş., Məmmədov Ə.Ç., Məmmədov E.R., Həsənov Ə.B., Hüseynova İ.M. Azərbaycan populyasiyasının STR markerlərlə tədqiqi: II. STR markerlərin allel strukturu əsasında digər populyasiyalarla müqayisəli analizi // АМЕА-nın Xəbərləri (biologiya və tibb elmləri), 2017, cild 72, №2, səh. 5-20. 16. Mustafayev N.Sh., Mammadov E.R., Huseynova F.R., Mammadov A.Ch., Hasanov A.B., Huseynova I.M. Population-genetic and comparative interpopulation studies of Azerbaijan population based on the 15 autosomal STR markers // Transactions of the Institute of Molecular Biology & Biotechnologies, ANAS, 2017, vol. 1, p. 72-79. 17. Shahmuradov I.A., Mustafayev N.Sh., Abdulazimova A.U., Guliyeva H.F. Challenges in computational identification of promoters // Transactions of the Institute of Molecular Biology & Biotechnologies, ANAS, 2017, v. 1, p. 67-71. 18. Huseynova I.,, Balakishiyeva G., Aliyeva D., Gurbanova U., Bayramova J., Mustafayev N., Aliyev J. Changes in the activities of metabolic enzymes and antioxidant defense system in 'Candidatus phytoplasma solani' infected pepper (Capsicum annuum L.) plants // Net Journal of Agricultural Science, 2017, v 5, No 2, p. 58-65. 19. Quliyeva H.F., Abduləzimova Ə.Ü., Mustafayev N.Ş., Şahmuradov İ.Ə. 7 Ali bitkinin zülal kodlaşdıran nüvə genlərinin potensial transkripsiya start saytlarının müəyyənləşdirilməsi // АМЕА-nın Xəbərləri (biologiya və tibb elmləri), 2017, c. 72, No 1, p. 14-22. 20. Mustafayev N.Ş., Məmmədov Ə.Ç., Məmmədov E.R., Həsənov Ə.B., Hüseynova İ.M. Azərbaycan populyasiyasının STR markerlərlə tədqiqi: I. STR markerlərin əsas populyasion-genetik parametrlərinin təyini. АМЕА-nın Xəbərləri (biologiya və tibb elmləri), 2016, cild 71, №2, səh. 5-16. 21. Əliyeva H.F., Abduləzimova Ə.Ü., Mustafayev N.Ş., Süleymanova L.M., Abaszadə Z.Ə., Şahmuradov İ.Ə. Ali Bitkilərin Plastid Təyinatlı Nüvə Genləri // АМЕА-nın Xəbərləri (biologiya və tibb elmləri), 2014, c. 69, No 2, p. P. 46-56. 22. Shahmuradov I., , Mustafayev N.Sh., Abdulazimova A., Akbarova Y., Zaib Khan F., Qamar R., Solovyev V., Aliyev J. The PlantProm DB: recent updates // Proceedings of the 2012 International Conference on Biomedical Engineering and Biotechnology (ICBEB), 2012, p. 1-3. 23. Shahmuradov I., Mustafayev N.Sh., Abdulazimova A., Akbarova Y., Zaib Khan F., Qamar R., Solovyev V., Aliyev J. Mitochondrial DNA insertions in Arabidopsis genome: is Organelle-to-nucleus gene transfer continued? // Proceedings of Aze4rbaijan NAS (biol. and med. sci.), 2012, c. 65, No 5-6, p. 184-194. 24. Новрузов Э.Н., Абдуллаева Г.А., Мустафаев Н.Ш., Расулов Ф.А. Флавоноиды надземной части Alhagi pseudoalhagi (Fabaceae) в Азербайджане. Раст. Ресурсы, 2010, т. 46, №4, c. 92-98. 25. Novruzov E.N., Abdullaeva G.A., Shamisizade L.A, Mustafayev N.Sh. Flavonoids and anthosyans from Alhagi pseudoalhagi // Chemistry of Natural Compounds, 2009, v. 45, No 2, p. 249-250. 26. Новрузов Э.Н., Абдуллаева Г.А., Шамсизаде Л.А., Мустафаев Н.Ш. Флавоноиды и антоцианы из Alhagi pseudoalhagi. Химия природных соединений (ХПС), 2009, №2, с. 213-214. 27. Новрузов Э.Н., Drsata J., Мустафаев Н.Ш., Раджабов А.А., Вагабов З.В. Флавоноиды надземной части Fagopyrum esculentus Moench. // Известия НАН Азербайджана, сер. биол. наук, 2009, v. 65, No 5-6, p. 9-13. 28. Mustafayev N.Sh., Sultanova F.A., Yusifov T.N. Population analysis of genetic distance by using RFLP of M13 phage // Proceedings of Azerbaijan NAS, ser. biol. sci., 2009, v. 64, No 1-2, p. 3-9. 29. Мустафаев Н.Ш., Шахмурадов И.А. Сателлитные ДНК: их организация и возможная роль в процессах регуляции генома. Труды Института Ботаники НАН Азербайджана, 2008, т. XXIX, с. 569-577. 30. Şahmuradov İ.Ə., Əkbərova Y.Y., Əbdüləzimova Ə.Ü., Mustafayev N.Ş. Qarğıdalının plastid genlərinin yeni promotor elementləri. AMEA Botanika İnstitutu-nun Elmi Əsərləri, 2004, c. XXV, s. 464-468. 31. Şahmuradov İ.Ə., Mustafayev N.Ş., Əkbərova Y.Y., Əbdüləzimova Ə.Ü. RNT polimeraza II ilə transkripsiya olunan bitki genlərinin promotorlarının məlumat bazası. http://www.rhul.ac.uk/; http://www.softberry.com; http://cub.comsats.edu.pk; 2003-2008. 32. Мустафаев Н.Ш. Сравнительный анализ 5'-промоторных районов генов clpP некоторых пластидных геномов высших растений. «Известия» НАН Азербайджана, сер. биол. наук., 2002, №1-6, с. 301-310. 33. Vəliyeva A., Mustafayev N.Ş., Şahmuradov İ. Nikotiana tabacum – tütünün plastid henlərinin bəzi potensial tənzimləyici elementləri. Azərbaycan Biokimyaçılar və Molekulyar Bioloqlar Cəmiyyətinin I Konfransı (məqalələr toplusu). Bakı, 2001, s. 59-61. 34. Shahmuradov I.A., Akberova Y.Yu., Mustafayev N.Sh., Abdulazimova A.U., Aliev J.A. Computer analysis reveals a set of additional promoter elements upstream of maize plastid genes. Proceedings of the Second International Conference on Bioinformatics of Genome Regulation and Structure, Novosibirsk, Russia, 2000, v. 1, pp. 142-144. 35. Shahmuradov I.A., Mustafayev N.Sh., Aliev J.A. Organization of plastid gene’ promoters. Proceedings of the 18th International Congress of Biochemistry and Molecular Biology, Birmingham, UK, 2000, p. 136. 36. Shahmuradov I.A., Mustafayev N.Sh., Aliev J.A. Some putative gene regulatory elements of transcription regulation of maize chloroplast genome. Proceedings of the 3rd International Conference on Molecular Structural Biology, Vienna, Austria, 1999, p. 153. 37. Shahmuradov I.A., Mustafayev N.Sh., Aliyev J.A. Some possible elements of transcription regulation of the rice chloroplast encoded genes. Proceedings of the First International Conference on Bioinformatics of Genome Regulation and structure, Novosibirsk-Altay Mountains, Russia, 1998, v. 2, pp. 438-441. 38. Shahmuradov I.A., N.Sh., Zulfugarov I.S. Comparative analysis of promoter regions of rice chloroplast encoded genes: possible elements of transcription regulation. Abstracts of 25th Silver Jubilee Meeting of the FEBS, The Bella Center, Copenhagen, Denmark, 1998, p. 154. 39. Shahmuradov I.A., Mustafayev N.Sh., Zulfugarov I.S. Comparative analysis of promoter regions of rice, tobacco and maize chloroplast genes. Abstracts of the XIth Congress on Photosynthesis, Budapest, Hungary, 1998, p. 153. 40. Shahmuradov I.A., Mustafayev N.Sh. Some possible elements of transcription regulation of the rice chloroplast encoded genes. Proceedings of the First International Conference on Bioinformatics of Genome Regulation and structure, Novosibirsk-Altay Mountains, Russia, 1998, v. 1, p. 176. 41. Шахмурадов И.А., Юсифов Т.Н., Мустафаев Н.Ш., Алиева Х.К., Аллахвердиева Е.А., Амикишиев В.Г. Некоторые особенности структурной организации и эволюции минисателлитов ДНК и их возможная роль в геноме. Azərbaycan Bitki Fizioloqlarının I Qurultayı (məqalələr toplusu), Bakı, 1997, s. 130-133. |
| Membership with international and foreign scientific organizations | Member of the Azerbaijan Society of Molecular Biology and Biochemistry
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| Pedagogical activity | Teaching "Molecular Evolution", "Mutagenesis and Reparation" and "Genomics" specialty courses at the master's degree level of education at the Institute of Molecular Biology and Biotechnology of ANAS |
| Other activities | Leading expert of the Molecular Genetics Laboratory in the Biology Department of the Public Legal Entity “Forensic Medical Expertise and Pathological Anatomy” of the Ministry of Health of the Azerbaijan Republic. |
| Awards and prizes | Honorary Decree of the Institute of Botany of Azerbaijan NAS (2015) Honorary Decree of the Institute of Molecular Biology and Biotechnology of Azerbaijan NAS (2020) |
| Place of work and its address | Institute of Molecular Biology and Biotechnologies, Azerbaijan NAS, AZ1073, Baku city, Izzat Nabiyev street 11 |
| Position | Leading Scientist |
| Office phone | (+994 12) 538 11 64 |
| Mobile | (+994) 70 327 57 76 |
| Home | (+994 12) 434 24 12 |
| Fax | (+994 12) 510 24 33 |
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