Name of the laboratory:

Molecular Bioengineering

Phone:

(+994 12) 436 90 05

Fax:

(+994 12) 510 24 33 

E-mail:

Molecbioenglab@imbb.science.az

Head of the laboratory:

Corresponding member of ANAS Tarlan Hazarpasha Mammadov

Staff:

junior researcher Aysu-Shovkat Mahammad Hasanova

junior researcher Aytaj Ismayil Murshudzade

Main activity directions:

Recombinant protein (vaccines, therapeutic proteins, antibodies, human and industrial enzymes, toxins, etc) production,

 Biofuels and bioenergy

Main scientific achievements:

Several therapeutic targets, vaccines, therapeutic proteins, and inhibitors against COVID-19 coronavirus have been successfully synthesized and developed in plants.

For the first time, functionally active, full-length Pfs48 / 45 protein of Plasmodium Falciparum, one of the leading candidates for the malaria vaccine was successfully synthesized in plants using Endo H enzymatic technology.

For the first time, a low-cost and highly immunogenic, stable and safe anthrax vaccine has been successfully synthesized in plants using Endo H enzymatic technology.

For the first time, a robust technology was developed by co-expression of target proteins of interest with bacterial Endo H enzyme, which enabled production of a number of valuable recombinant proteins (vaccines, therapeutic proteins, enzymes etc.) in plant leaves in non-glycosylated form without amino acid change in the resulting protein. Using these technology vaccines for anthrax (for human and veterinary use) and malaria (for human use) have been successfully produced in green plant leaves.

For the first time, an expression system technology has been developed that provides glucose-free protein synthesis in eukaryotic systems, including the plant system. This technology has enabled the synthesis of very important and difficult-to-express complex protein antigens, vaccines and therapeutic proteins with high activity.

For the first time, the presence of glyco-proteins consisting of a residue of sialic acid combined with 1.4 galactose was discovered in green algae that fix carbon dioxide.

The enzyme phosphoglycolophosphatase, the main enzyme of photorespiration, was first obtained in the pure form from a eukaryotic organism, which allowed establishing the gene structure of the enzyme for the first time and to identify it in other organisms, including humans.

New genes and catalytic subunits of PEP-carboxylase, a key enzyme of C4 photosynthesis have been discovered in C. reinhardtii green alga.

The fastest gene synthesizing biotechnology in the world has been developed.

For the first time, the annealing time for the successful amplification of high guanine/cytosine-containing DNA was established.

For the first time, the enzyme carbonic anhydrase was purified from the Cicer arietinum plant, crystallized and examined under an electron microscope, and the enzyme was shown to have an octameric structure.