| Florida Radik Mehraliyeva | |
| Place of birth | Azerbaijan, Khachmaz region, Yalama |
| Date of birth | 01.11.1977 |
| Education | Baku State University, Faculty of Physics |
| Scientific degree | PhD in Biology |
| Academic rank | Assiciate Professor |
| Speciality code and title for PhD thesis | 2406.01
Biophysics
“Oxidative-destructive processes induced in erythrocytes with different selenium status” |
| Total number of printed scientific publications | 55 |
| Number of scientific publications printed abroad | 34 |
| Number of papers published in journals indexed and abstracted in international databases | 8 |
| Number of patents and certificates of authorship |
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| Staff training: - number of PhD |
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| Key scientific achievements | 1. It has been established that the initial link in the induced oxidation of erythrocytes is the oxidative modification of haemoglobin (Hb) (accumulation of methaemoglobin, etc.), which precedes oxidative hemolysis. The lag periods of methaemoglobin accumulation are ≈ 2 times shorter than the lag period of hemolysis. 2. It has been shown that the supply of selenium in erythrocytes plays a significant role in the regulation of oxidative reactions. Exogenous enrichment of erythrocytes with selenium (parenteral introduction of sodium selenite - 0.5 mg/kg) enhances the oxidative resistance of animal erythrocytes by increasing GPx activity and due to the enrichment of haemoglobin with selenium, and selenium deficiency (model - blood of pregnant women) leads to intensified lipid peroxidation of erythrocytes and accelerated accumulation of MetHb. 3. The distribution of intraerythrocyte selenium is species-specific. In rats, approximately 75% of selenium is contained in the GPx fraction, and <25% is contained in the Hb fraction. For guinea pigs and humans, the opposite is true: 15-30% is contained in the GPx fraction and 70-85% is contained in the Hb fraction. The total selenium content in rat erythrocytes is >1.5 times higher than its concentration in human and guinea pig erythrocytes. 4. An assessment of the current selenium status in Azerbaijan (using Baku as an example) revealed a significant decrease in selenium status among residents of Azerbaijan compared to the 1970s: ≈90 μg/L versus ≈120 μg/L in the blood. A close correlation (r≈0.71) was established between selenium content and the activity of the selenoenzyme glutathione peroxidase in the blood, providing indirect evidence of low selenium status among residents of the region. 5. It has been shown that during pregnancy, the selenium content in the blood of women in Azerbaijan decreases to a critical value (≈ 40 μg/l), i.e. ≈ 2 times, and the activity of GPx in erythrocytes decreases by no more than 30-50%, which indicates the need to monitor selenium status by directly measuring its content in whole blood and the feasibility of dietary selenium correction. 6. During pregnancy, a decrease in selenium content (up to ≈ 2 times) was noted in the haemoglobin fraction of erythrocyte lysates, which was accompanied by a decrease in quasi-glutathione peroxidase activity (up to 30%) of the haemoglobin fraction, which indicates the presence of a certain relationship between these indicators. 7. Oxidative stress induced by sodium nitrite has a dose-dependent effect on the activity of the main antiperoxide enzymes catalase and glutathione peroxidase. As the concentration of sodium nitrite increases, the activity of catalase decreases monotonically. However, for GPx there is a two-phase character change in the activity: low doses stimulate an increase in GP; high - a decrease. 8. The changes in GPx activity negatively correlate with intensification in erythrocytes lipid peroxidation, that indicates the dominant role of GPx selenenzyme and selenium as an endogenous antioxidant in the regulation of erythrocyte lipide peroxidation under nitrite exposure. 9. The use of sodium selenite in a final concentration of 5μM in an incubation medium containing erythrocytes as a possible antioxidant leads in all cases to a decrease in the oxidative effects of sodium nitrite: a decrease in the intensity of lipid peroxidation of erythrocytes, the accumulation of MetHb, a decrease in the inhibition of GPx and catalase activity. 10. The incorporation of selenium (sodium selenite, 10 μM) into haemoglobin slows the process of its oxidative modification (depletion of oxyhemoglobin, accumulation of methaemoglobin and ferrylhaemoglobin), resulting in a noticeable (20-30%) reduction in fluorescence peaks reflecting the oxidative degradation of heme in the absence of antioxidant enzymes activity (catalase, glutathione peroxidase, and peroxiredoxin-2) that utilize hydrogen peroxide. This indicates that selenium can act as a free antioxidant in haemoglobin. |
| Titles of publications | 1. Т.М. Гусейнов, Ф.Р. Яхъяева, Р.Т. Гулиева. Протекторное действие селена от неблагоприятного фактора окружающей среды // Reproduktiv sağlamlıq və perinatologiya, 2005, № 3, с. 48-52. 2. Ф.Р. Яхъяева, Т.М. Гусейнов. Действие электрических полей высокой напряженности на эритроциты с пониженным статусом селена // Экоэнергетика, 2007, № 2, с. 38-40. 3. Р.Т. Гулиева, Ф.Р. Яхъяева, Т.М. Гусейнов. Озон как инициатор окислительных процессов в клетках красной крови // Биомедицина, Баку, 2008, № 2, с. 30-33. 4. Р.Т. Гулиева, Ф.Р. Яхъяева. Особенности индуцированного озоном окисления эритроцитов животных с различным метаболизмом селена // Известия, серия биологических наук, 2008, №5-6, с.179-183. 5. Т.М. Гусейнов, Ф.Р. Яхъяева, Р.Т. Гулиева. Влияние селена на устойчивость гемоглобина к фотоокислительным процессам // Украинский биохимический журнал, 2012, т. 84, № 2, стр. 53-60. 6. Т.М. Гусейнов, Ф.Р. Яхъяева, Э.С. Багирова, Р.Т. Гулиева. Селен и проблема репродуктивного женского здоровья // Микроэлементы в медицине, 2012, т. 13, № 1, с. 25-28. 7. Т.М. Гусейнов, Р.Т. Гулиева, Ф.Р. Яхъяева, Э.Д. Багирова. Пероксидазная активность гемоглобина (в присутствии GSH) в лизатах эритроцитов беременных женщин с Г-6-ФД дефицитом при воздействии электрического поля высокой напряженности // Электронный журнал «Вестник Новосибирского государственного педагогического университета». Новосибирск, 2013, 6(16), с.106-114. 8. Т.М. Гусейнов, Ф.Р. Яхъяева. Селен и старение, роль селена в геронтологических процессах // Биомедицина, № 4, 2015, с. 3-7. 9. С.Я. Гусейнова, Р.Т. Гулиева, М.З. Дадашов, А.И. Джафаров, Ф.Р. Яхъяева, Т.М. Гусейнов. Окислительная модификация гемоглобина изолированных эритроцитов в инкубационной среде, содержащей нитрит натрия и селенит натрия // Электронный журнал «Вестник Новосибирского государственного педагогического университета». Новосибирск, 2016, 5(33), с. 207-217. 10. С.Я. Гусейнова, Т.М. Гусейнов, Р.Т. Гулиева, Ф.Р. Яхъяева, М.З. Дадашов, А.И. Джафаров. Регуляция селеном окислительных процессов в крови крыс, индуцированных нитритом натрия // Микроэлементы в медицине, 2017, т. 18, № 4, с. 13-17. 11. С.Я. Гусейнова, Р.Т. Гулиева, Ф.Р. Яхъяева, Т.М. Гусейнов. Проблема йоддефицита в Азербайджане. Роль микроэлемента селена в регуляции метаболизма йода // Биомедицина, 2019, № 2, с. 4-12 12. Т.М. Гусейнов, Р.Т. Гулиева, Ф.Р. Яхъяева. Биологическое значение селена и его место при РНК вирусных заболеваниях // Микроэлементы в медицине. 2020, 21(4), с. 21-31. Импакт-фактор РИНЦ: 0,828 (DOI: 10.19112/2413-6174-2020-21-4-21-31) (https://journal.microelements.ru/uploads/2020_4/21_31_21(4)_2020.pdf) 13. T.M. Huseynov, S.Y. Huseynova, R.T. Guliyeva, M.Z. Dadashov, S.М. Rahmanova (Maharramova), F.R. Yakhyayeva, S.А. Jafarova, Characteristics of oxidative stress induced by moderate doses of sodium nitrite in isolated erythrocytes in the presence of sodium selenite // Микроэлементы в медицине. 2021. 22(2):25-35; Импакт-фактор РИНЦ: 0,828 (https://journal.microelements.ru/uploads/2021_2/25_35_22(2)_2021.pdf) 14. Т.М. Гусейнов, С.М. Рахманова, Ф.Р. Мехралиева, Г.Я. Абыев. Селен и оксид азота в гемоглобине «друзья» или соперники? // Azərbaycan təbabətinin müasir nailiyyətləri. 2023, № 2, c. 262-266. 15. Т.М. Гусейнов, С.М. Рахманова, Ф.Р. Мехралиева. Селен как протектор от пероксидводородной окислительной деградации гема гемоглобина без участия глутатионпероксидазного механизма // Biophysics (Russian Federation), 2025, т. 70, №1, с. 104-111. CiteScore 1.2, Scopus, Q4. https://doi.org/10.1134/S0006350925700125 |
| Membership in national, international, and foreign scientific organizations |
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| Pedagogical activity | MSE RA Institute of Physics, MSE RA Institute of Biophysics |
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| Place of work and its address | Ministry of Science and Education of the Republic of Azerbaijan “Institute of Molecular Biology” PLE, AZ1073, Baku, Izzet Nabiyev Street, 11 |
| Position | senior researcher |
| Office phone |
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| Mobile | +994559159335 |
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| | fl.yahyayeva@gmail.com |
