Name of the laboratory:

Plant Biotechnology

Phone:

(+994 12) 538 11 64

Fax:

(+994 12) 510 24 33 

E-mail:

pbiotechnologylab@imbb.science.az

Head of the laboratory:

Doctor of Philosophy in biology Mahira Haybat Mammadova

Staff:

Ph.D., assoc.professor, leading researcher Sadagat Shirmammad Asadova

junior researcher Elnara Etibar Sadigova

junior researcher Gunay Ilman Ismayilova

junior researcher Aygün Aghaverdi Sadigova

Main activity directions:

Study of the fundamental principles of in vitro induction of embryogenesis and morphogenesis in cereal plants;

Development of saffron propagation technology. (Crocus S. L);

Using the example of the indigenous grape variety Shirvan-Shakhi, the elaboration, and development of microclonal reproduction technology to save the gene pool of rare and endangered plant species;

Cell selection. Obtaining forms with high adaptive capacity and resistance to abiotic stress by in vitro methods;

Micropropagation of some commercial rose varieties (Rosa L.)

Main scientific achievements:

Isolated cells from mature wheat germs were obtained and plant reconstruction in vitro was implemented. A method for gas-chromatographic determination of respiratory gas metabolism of fetuses was developed directly on a maternal plant and consistently from a single sample, with the usage of gas-liquid and gas-adsorption chromatography, hydrocarbon and low-boiling gases were separated. The hormonal role of ethylene in the aging of plant tissues was studied. A model apple cell culture system was created and it was shown that the in vitro model system adequately reflects certain biochemical sequences in the ontogenesis of fruits. The resulting culture was cultivated on an artificial medium for 7 years. With the usage of methods of cellular engineering, regenerative plants from callus cells of 14-day-old embryos were obtained. Forms of wheat with somaclonal variability were obtained for use in breeding practice. The effect of increased temperature on morphogenetic and adaptive processes in the culture of wheat cells in vitro was studied. The deterministic role of ethylene in the adaptive ability of a genotype was established. The possibility of obtaining plants and selecting strains of cells possessing increased resistance to temperature was shown. A foreign gene was transferred to tobacco plants and a transgenic plant was obtained with marker signs of kanamycin resistance. The technology of clonal multiplication has been developed for 10 valuable table grapes. An isolated culture of ginseng cells (Panax Ginsenq) was obtained. A strain with high pharmacological activity was obtained. The ginseng cell strain was cultivated for 15 years. Questions related to the problems of the correlation of cell and organismic resistance in wheat with the use of signal systems at the in vitro and in vivo level were studied. The idea of resistance to salt stress is formulated as the existence of a cascade system of protective reactions. The idea of resistance to salt stress was formulated as the existence of a cascade system of protective reactions. The technology of accelerated multiplication of vines from one-eyed stalks with application of hormonal factors was presented. The technology was applied in practice. A system of plant cultivation based on agaroponics has been developed, which makes it possible to identify plant resistance and the degree of manifestation of adaptivity in the gradient of an increasing stress effect. The technology of saffron breeding was developed. (Crocus S. L). The results obtained have no analogs. From 10 to 25 corms were obtained from slices of corms in vitro by induction of morphogenesis at the stage of organogenesis. Studies were conducted to save the gene pool and restore the population of the aboriginal grape variety Shirvan-Shchakhi. A technology for biotechnological propagation of this variety has been developed. For the first time, the possibility of passaging isolated saffron cells with preservation of morphogenic and embryogenic ability was shown. For the first time, groups of antibiotics were studied to reduce the colonization of the Shirvan-Shahi grape plant by endogenous microflora. To determine the concentration dependences in vitro chemotherapy studies, antibiotics were selected and the level of their toxic effect on the growth processes of the Shirvan Shahi grape variety in vivo was studied.