HomeLaboratory of Bioadaptation sa sa

Name of the laboratory:

Bioadaptation

Phone:

(+994 12) 5381164

Fax:

(+994 12) 5102433

E-mail:

i.huseynova@imbb.science.az

bioadaptationlab@imbb.science.az

Head of the laboratory:

Academician Irada Mammad Huseynova

Staff:

Ph.D. in biology, assoc.professor, leading researcher Samira Mahammadrahim Rustamova

Ph.D. in biology, leading researcher Gulnara Shammad Balakishiyeva

doctor of biological sciences, assoc.professor, leading researcher Tofig Idris Allahverdiyev

Ph.D. in biology, assoc.professor, leading researcher Nargiz Fakhraddin Sultanova

Ph.D. in biology, senior researcher Samra Tahir Mirzayeva

researcher Turana Yashar Isgandarova

researcher Lala Museyib Aydinli

researcher Saida Tofig Zulfugarova

junior researcher Nargiz Kamal Bayramova

junior researcher Shahriyar Fateh Sadigov

junior researcher Gulnar Rizvan Abdullayeva 

junior researcher Aysel Mushfig Madadli

junior researcher Ravane Feyzulla Maliyeva

Main activity directions:

Determination of the molecular basis of adaptive processes of abiotic and biotic stresses, investigating the possibility of genetic improvement of stress tolerance.

Main scientific achievements:

Molecular-genetic mechanisms of the synthesis, assembly and formation of the major structural-functional components - pigment-protein complexes of photosynthetic membrane in higher plants are comprehensively investigated; probable role of photosynthetic changes in plant adaptation to extreme conditions of growing is determined and molecular bases of the defense-adaptive processes of plants to abiotic (drought) and biotic (virus, phytoplasma, rust) stress factors are identified.

The mechanisms of wheat adaptation to drought were identified and the tolerant genotypes were evaluated for selection as a result of the study of free radicals formation, antioxidant defense systems, native and functional forms of chloroplasts, synthesis of thylakoid membrane proteins in all phases of the ontogenesis of wheat genotypes cultivated in field conditions under water shortage, and on the basis of screening for drought tolerance of the durum (Triticum durum Desf.) and bread (Triticum aestivum L.) wheat genotypes collected in the Gene Pool of Azerbaijan using RAPD, SSR and functional markers;

The effective genes of tolerance to brown, yellow and stem rust are revealed as a result of the analysis of wheat genotypes using STS, SCAR and SSR molecular markers and KASP genotyping technology;

Race composition in the causative of the stem rust disease has been molecularly characterized in wheat plants from various regions of Azerbaijan. SNP (Single nucleotide polymorphism) genotyping based on single nucleotide polymorphism has not revealed the Ug99 race group. Genetic diversity has been observed in 54 samples of 17 multilocus genotypes. It has been found that 12 samples belong to TKTTF race group, whereas 42 samples belong to a new, unidentified race group;

RNA- and DNA-genome viruses and their mixes were identified for the first time in various agricultural and wild vegetable plants collected from different regions in Azerbaijan using serological and molecular analysis; their molecular identification was carried out and biochemical, physiological and biophysical characteristics of infected plants were studied;

During a joint research of nanovirus diseases of cultivated and wild beans, conducted in several European countries, a new nanovirus-Black medic leaf roll virus (BMLRV) was identified and its complete genome was sequenced in chick-pea plants collected in Ganja. As a result of the complete sequencing of five isolates of Faba bean necrotic yellow virus (FBNYV) in bean samples from Azerbaijan and Spain and four isolates of Faba bean necrotic stunt virus (FBNSV) in chick-pea, green pea, lentils and bean samples from Azerbaijan it was proved that the FBNSV nanovirus was identified in Europe for the first time;

 

For the first time in Azerbaijan nucleotide sequence of citS gene encoding malate/citrate simporter protein was established using the "Chromosome walking" method and a new Nested PCR method was developed to determine the Stolbur group (XII).   For the first time to our knowledge evidenced the existence of inter-species recombination between ‘Ca. P. pyri’ and ‘Ca. P. prunorum’ on pear tree in Azerbaijan;

 

For the first time in Azerbaijan as a result of the 16S ribosomal gene sequence analysis was detected 'Candidatus Phytoplasma solani' which causes dangerous ‘Bois Noir’ disease in grapevine, genotyping of detected phytoplasma isolates by multi-locus sequence analysis revealed 4 new genotypes for Stamp and 3 new genotypes for secY gene;

 

In Azerbaijan “Hyalestes obsoletus” and “Reptalus noahi” were identified as insect vectors of the 'Candidatus Phytoplasma solani' by   barcoding based on mtCOI mitochondrial marker.  This is first report of “Reptalus noahi” as insect vector spreading "Candidatus Phytoplasma solani";

Sequence of non-ribosomal stamp-gene from pepper (Capsicum annuum) AZ2012-PV2 from Azerbaijan was identified the mixture of two stolbur isolates of phytoplasma differing by single nucleotide polymorphism (SNP);

For the first time tomato yellow leaf curl virus (TYLCV) was detected in a tomato plant and the virus isolate was uploaded in GenBank under registration number KY412552

For the first time Watermelon mosaic virus (WM) belonging to the Potivirus family was identified in the Cucumis melo L. as a result of sequential analysis of amplification products synthesized with specific primer pairs to the C-terminus of RdRp and Nib-proteins of the viral genome and the N-terminus of the capsid protein-CP (registration numbers in Genbank: MG964114-MG964228). At the same time, recombination between groups G2/G1 was found in WMV isolates;

The DREB transcription factor gene was isolated and molecularly characterized from the genome of durum wheat genotype Barakatli-95. Three conserved regions specific for DREB gene identified in sequence products: the EREBP/AP2 DNA-binding domain, the N-terminating signal that determines its localization in the nucleus, and the Ser/Thr rich region adjacent to the EREBP/AP2 domain;

A comparative analysis of drought-induced DREB1 transcription factor gene expression in wheat genotypes of Azerbaijani and German origin was performed.